Efficient bioconversion of compactin to pravastatin by the quinoline-degrading microorganism Pseudonocardia carboxydivorans isolated from petroleum-contaminated soil.
نویسندگان
چکیده
Pravastatin is one of the first available statins on the market. The purpose of this study was to isolate and identify the quinoline-degrading microorganism from petroleum-contaminated soil that could bioconvert compactin to pravastatin. There were 10,011 microorganism colonies isolated; five strains showed a higher capability for quinoline biodegradation. These five strains were evaluated for their pravastatin bioconversion ability; Pseudonocardia sp. had the highest efficiency for conversion of compactin to pravastatin. The strain was further identified as Pseudonocardia carboxydivorans PAH4. The bioconversion rates were studied under difference incubation conditions. Pre-incubation in medium containing 0.005% compactin sodium, resulted in the compactin utilization rate of almost 100% in a 1mg/ml compactin-containing medium. The rate of conversion of pravastatin was up to 68% after 6 days of incubation. In conclusion, the results of this study suggest that P. carboxydivorans PAH4 could be considered a candidate for the production of pravastatin on an industrial scale.
منابع مشابه
Characterization of Bacteria Degrading Petroleum Derivatives Isolated from Contaminated Soil and Water
متن کامل
Pseudonocardia carboxydivorans in human cerebrospinal fluid: a case report in a patient with traumatic brain injury
BACKGROUND Members of the genus Pseudonocardia have been widely reported and recovered from several ecosystems, such as soil samples and plant samples. Pseudonocardia bacteria colonize the microbial communities on the integument of fungus gardening ant species. We present the first documented case of Pseudonocardia carboxydivorans isolated in human cerebrospinal fluid (CSF). To the best of our ...
متن کاملAnthracene degradation by an oligotrophic bacterium isolated from refinery soil
Anthracene is a widespread environmental pollutant with carcinogenic and genotoxic properties. Biodegradation is a simple, cost-effective and safe technique to clean-up contaminated environments. The aims of this study are isolation and characterization of an oligotrophic bacterium with the ability to degrade anthracene and the assessment of in vitro biodegradation process. For this purpose pet...
متن کاملارزیابی باکتری های جدا شده از لجن فعال حاصل از تصفیه فاضلاب شهری منطقه ویژه عسلویه جهت زیست فزونی خاک های آلوده به کروزن
Background and Objectives: Bioaugmentation is a superior technique in bioremediation of contaminated soils with petroleum hydrocarbons. The aim of this study was to evaluate the effect of isolated bacteria from activated sludge of Asalouyeh special zone municipal wastewater treatment for bioaugmentation of kerosene-contaminated soils and to study the growth of isolated bacteria in the presence ...
متن کاملStudy of Adaptations in Bacterial Isolates Inhabiting Petroleum Contaminated Soil
Oil spills and petroleum based pollutions are very common which cause a potential threat to both terrestrial as well as aquatic life. Bioremediation has become an alternative way to remedy. These microorganisms can degrade a wide range of target constituents present in oil sludge. An ability to isolate high numbers of certain oil degrading microorganisms from an environment is commonly taken as...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bioresource technology
دوره 102 22 شماره
صفحات -
تاریخ انتشار 2011